EFFECT OF GROUND AND NON-GROUND BAY LEAF (Syzygium polyanthum) ON AORTIC THICKNESS IN WISTAR RATS
DOI:
https://doi.org/10.33992/meditory.v14i1.4876Keywords:
Ground and non-ground bay leaf, Aortic thickness, Wistar rat, HypercholesterolemiaAbstract
Background: Hypercholesterolemia promotes aortic wall thickening and increases cardiovascular risk. Although Syzygium polyanthum (bay leaves) has antioxidant and hypocholesterolemic properties, evidence comparing crushed and non-crushed decoctions remains limited.
Objective: This study aimed to compare the effects of crushed and non-crushed bay leaf decoctions on aortic wall thickness in hypercholesterolemic Wistar rats (Rattus norvegicus).
Methods: A randomized post-test only control group study was conducted using 40 male Wistar rats assigned to eight groups. Bay leaf decoctions (crushed or non-crushed; 0.52%, 0.72%, and 0.92%) were administered orally for 14 days. Aortic wall thickness was assessed histologically and analyzed using the Kruskal–Wallis and Dunn's tests.
Results: A significant difference in aortic wall thickness was observed among the groups (p = 0.040). The lowest mean aortic wall thickness was observed in the 0.92% crushed bay leaf decoction group (92.00 ± 29.20 μm), which was lower than that of the hypercholesterolemic control group (188.00 ± 29.24 μm).
Conclusions: Crushed bay leaf decoction, especially at 0.92%, more effectively reduced aortic wall thickness than the non-crushed preparation. Further studies are needed to confirm the underlying mechanisms.
References
1. Christina TY, Andika IPJ, Saffarudin, Yulianti TS, Sriwiyati L, Oktavia YD, Fatimah K. Peningkatan kesehatan masyarakat: upaya pencegahan dan pengendalian penyakit kardiovaskuler melalui deteksi dini kadar kolesterol. Global Abdimas: Jurnal Pengabdian Masyarakat. 2025;5(1):36–42. doi:10.51577/globalabdimas.v5i1.664
2. Qatrunnada S, Amalia AA, Ismarwati. Hubungan kadar kolesterol total dengan tekanan darah pada pasien penyakit jantung koroner usia pra lansia di RSUD Linggajati Kabupaten Kuningan. Jurnal Kesehatan Tambusai. 2024;5(September):9695–9704.
3. Santoso A, Citraningtyas T, Viora E, Gotera W, Fujiati II, Lukito W, Vidiawati D, Claramita M, Diatri H, Prawira B, Grace A, Arsita E, Billy A. Towards integrated cardiovascular and mental health management in primary health care in Indonesia: a policy outlook. Lancet Reg Health Southeast Asia. 2025 May 27;37:100605. doi:10.1016/j.lansea.2025.100605. eCollection 2025 Jun.
4. Suarsih C. Hubungan pola makan dengan kejadian kolestrol pada lansia di wilayah kerja Puskesmas Tambaksari. Jurnal Keperawatan Galuh. 2020;2(1). Available from: https://jurnal.unigal.ac.id/index.php/JKG
5. Maryanto S, Oktianti D. Apo-B levels and abdominal aortic wall thickness in hypercholesterolemic rats treated with red guava fruit: Kadar Apo-B dan ketebalan dinding aorta abdominalis tikus hiperkolesterolemia dengan perlakuan buah jambu biji merah. Amerta Nutrition. 2023;7(1):112–119. doi:10.20473/amnt.v7i1.2023.112-119. Available from: https://ejournal.unair.ac.id/AMNT
6. Isdadiyanto S. Tebal dinding dan diameter lumen arteria koronaria tikus putih setelah pemberian teh kombucha kadar 100% waktu fermentasi 6, 9 dan 12 hari. Buletin Anatomi dan Fisiologi. 2018;3(1):97–104. Available from: https://ejournal2.undip.ac.id/index.php/baf
7. Dwijayanti I, Wilujeng CS, Soemardini. Pengaruh jus terong ungu terhadap ketebalan dinding aorta pada tikus putih dengan diet aterogenik. Jurnal Teknologi Pangan dan Gizi (Journal of Food Technology and Nutrition). 2021;20(2):124–129.
8. Emilia. Efektivitas pemberian rebusan daun salam terhadap kolesterol total pada penderita hiperkolesterolemia di wilayah kerja Puskesmas Desa Gedang, Kota Sungai Penuh, Provinsi Jambi tahun 2022. Jurnal Multidisiplin Dehasen. 2023;2(2):287–294.
9. Supartiningsih S, Hartati N. Uji aktivitas ekstrak etanol daun salam (Syzygium polyanthum (Wight.) Walp.) terhadap kadar kolesterol HDL dan LDL pada tikus putih jantan. Jurnal Review Pendidikan dan Pengajaran. 2024;7(4):18015. Available from: http://journal.universitaspahlawan.ac.id/index.php/jrpp
10. Widiyono W, Aryani A, Herawati VD. Pemberian air rebusan daun salam (Syzygium polyanthum) dapat menurunkan kadar kolesterol pada lansia dengan hiperkolesterolemia. Jurnal Kesehatan Holistik. 2021 Mar;15(1):39–47. Available from: http://ejurnalmalahayati.ac.id/index.php/holistik. doi:10.33024/hjk.v15i1.3351
11. Nurlely N, Putra AMP, Nurrochmad A, Widyarini S, Fakhrudin N. Extraction, phytochemicals, bioactivities, and toxicity of Syzygium polyanthum: A comprehensive review. J Herbmed Pharmacol. 2024;13(3):366–380. doi:10.34172/jhp.2024.51454.
12. Iriani Y, Ramona Y, Astiti NPA. Potensi ekstrak etanol daun salam dan air rebusan daun salam untuk memperbaiki profil lipid (LDL-kolesterol) darah pada tikus Wistar. J Metamorfosa J Biol Sci. 2021;8(1):89–98. doi:10.24843/metamorfosa.2021.v08.i01.p09.
13. Widyawati T, Sari MI, Pratiwi D, et al. Syzygium polyanthum ethanol extract decreased malondialdehyde level in type 2 diabetic patients. Pharmacogn J. 2021;13(5):1205–1210.
14. Pan F, Li X, Tuersuntuoheti T, Zhao L, Liu M, Fang X, Peng W, Tian W. Self-assembled condensed tannins supramolecular system can adsorb cholesterol micelles to promote cholesterol excretion. Int J Biol Macromol. 2024;262:131874. doi:10.1016/j.ijbiomac.2024.131874.
15. Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018;13:20. doi:10.1186/s13020-018-0177-x.
16. Bryda EC. The mighty mouse: the impact of rodents on advances in biomedical research. Mo Med. 2013;110(3):207–211.
17. Kamilah N, Hakim R, Purnomo Y. Efek ekstrak etanol daun gedi merah (Abelmoschus manihot (L.) Medik) terhadap persentase interleukin-10 (IL-10) dan sel T sitotoksik (CD8⁺) tikus model diabetes tipe II. J Kesehatan Islam. 2023;25(1):25–32.
18. Jannah BM, Isdadiyanto S, Sitasiwi AJ. Histopathological of white rats aorta induced by high-fat feed after administered by neem leaf ethanolic extract. Biosaintifika J Biol Biol Educ. 2020;12(3):370–8. doi:10.15294/biosaintifika.v12i3.25494
19. Anggraeny O, Venesa K, Magdalena N, Dame S, Winnie S, Nesya S, Nurdiana. Comparison of the effectiveness of lime [Citrus aurantifolia (Christm.) Swingle] and lemon [Citrus limon (L.) Burm.f.] juice to prevent lipid profile aberration. Indones J Hum Nutr. 2020;7(2):75–84.
20. Hijriani BI, Atfal B, Kodariah L, Hadiatun N, Ismatullah NK. Efektivitas ekstrak daun salam (Syzygium polyanthum) dalam mencegah kenaikan kadar kolesterol LDL tikus putih (Rattus norvegicus) diinduksi kuning telur puyuh. J Kesehatan Rajawali. 2023;13(2):1–4. doi:10.54350/jkr.v13i1.156
21. Hasanah N, Lubis TAF. Pengaruh jus buah pare terhadap kadar kolesterol HDL pada tikus putih galur Wistar yang diinduksi pakan tinggi lemak. J Pandu Husada. 2024;5(1):1–7. Available from: https://jurnal.umsu.ac.id/index.php/JPH
22. Aguwa US, Okeke SN, Nnabuihe ED, Eze CE, Azurunwa O, Obinwa BN, Ogbo OF, Obi KC, Onwuelingo S, Okonkwo D, Ogbuokiri D, Okeke C. Evaluating the effect of chloroform inhalation as a method of euthanasia on the cerebellum and hippocampus of adult Wistar rats. J Adv Med Pharm Sci. 2020;22(6):14–25. doi:10.9734/JAMPS/2020/v22i630176.
23. Anggraeny. Comparison of The Effectiveness of Lime [Citrus aurantifolia (Christm.) Swingle] and Lemon [Citrus limon (L.) Burm.f.] Juice to Prevent Lipid Profile Aberration. 2015;3(2):113–9.
24. Ningrum EWC, Isdadiyanto S, Mardiati SM. Histopatologi pankreas tikus putih (Rattus norvegicus L.) yang diberi pakan tinggi lemak dan paparan ekstrak etanol daun mimba (Azadirachta indica A. Juss). Buletin Anatomi dan Fisiologi. 2020;5(2):129–136.
25. Adiputra IMS, Trisnadewi NW, Oktaviani NPW, Munthe SA, Hulu VT, Budiastutik I, Faridi A, Ramdany R, Fitriani RJ, Tania POA, Rahmiati BF, Lusiana SA, Susilawaty A, Sianturi E, Suryana. Metodologi penelitian kesehatan. Watrianthos R, Simarmata J, editors. Medan: Yayasan Kita Menulis; 2021.
26. Swarjana IK. Metodologi penelitian kesehatan: edisi terbaru. Yogyakarta: Andi Offset; 2023.
27. Wibowo T, Maulani Y. Perbedaan hasil pewarnaan Hematoxylin-Eosin preparat limfonodi pada proses clearing menggunakan xylol dan minyak zaitun. Plenary Health: Jurnal Kesehatan Paripurna. 2024;1(3):197–201.
28. Handayani D, Febrianingsih E, Kurniawati AD, Kusumastuty I, Nurmalitasari S, Widyanto RM, Oktaviani DN, Innayah AM, Sulistyowati E. High-fructose diet initially promotes increased aortic wall thickness, liver steatosis, and cardiac histopathology deterioration, but does not increase body fat index. J Public Health Res. 2021;10:2181.
29. Sendy AM, Widodo A. Pengaruh ekstrak daun belimbing wuluh (Averrhoa blimbi L.) terhadap kadar kolesterol LDL serum tikus Wistar (Rattus norvegicus) hiperkolesterolemia. J Kedokteran Diponegoro. 2019;8(2):642–52.
30. Senawong K, Katekaew S, Juntahum S, Laloon K. Impact of grinding and sorting particle size on phytochemical yield in Dipterocarpus alatus leaf extract. Int J Biomater. 2023;2023:4512665. doi:10.1155/2023/4512665.
31. Susyani, Zurio A, Terati. The Bay Leaves Tea Can Decrease Cholesterol Levels of Patients with Cardiovascular Disease. World Journal of Advanced Healthcare Research. 2020;4(3):173-177.
32. Sakaganta ARI, Sukohar A. Daun salam (Syzygium polyanthum) sebagai penurun kadar kolesterol dalam darah. Medula. 2021;10(4):618.
33. Ismail A, Ramli NS, Mohamed M, Ahmad WANW. Acute and sub-acute antihypertensive effects of Syzygium polyanthum leaf extracts with determination of gallic acid using HPLC analysis. Pharmacogn J. 2018;10(4):663–71. doi:10.5530/pj.2018.4.109.
34. Salim HM, Kurnia LF, Bintarti TW, Handayani. The effects of high-fat diet on histological changes of kidneys in rats. Biomol Health Sci J. 2018;1(2):109–14. doi:10.20473/bhsj.v1i2.9675.
35. Anggrek TM, Mukono IS, Rochmanti M, Wungu CDK. Efficacy of bay leaf (Syzygium polyanthum) in regulating lipid profile in dyslipidemia model rats: a systematic review. Curr Intern Med Res Pract Surabaya J. 2024;5(1):31–8
36. Hasan R, Siregar GA, Lindarto D. The effect of bay leaf extract (Syzygium polyanthum) on vascular endothelial growth factor (VEGF) and CD31 (PECAM-1) expression in acute coronary syndrome. Medicinski Glasnik (Zenica). 2020;17(2):321–7. doi:10.17392/1208-20.
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