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Email this article GROWING Plasmodium berghei ANKA (PbA) FROM FROZEN AND FRESH DONORS: A COMPARATIVE STUDY
Abstract
Background: Plasmodium berghei is a rodent malaria parasite that infects mice and rats that mimics Plasmodium falciparum. It has a problem with standardized growing time and reaching high percentages of parasitemia, which allows optimum conditions in antimalarial research.
Objective: This study aimed to analyze different outcomes from growing P. berghei ANKA (PbA) from frozen compared with fresh donors.
Methods: The study involved 20 mice, with 10 infected using fresh and 10 using frozen P. berghei inocula. Blood smears were prepared daily for 7 days, fixed with methanol, stained with Giemsa, and examined every 24 hours at 1000× magnification. Parasitemia was calculated as infected erythrocytes per 1000 cells, recorded from initial parasite detection, and analyzed using a t-test.
Results: Parasite growth in mice inoculated with fresh and frozen P. berghei blood was monitored through parasitemia levels over 7 days. In the fresh blood group, parasites appeared on day 2 (0.8%) and increased steadily to 11.3% by day 7. In contrast, parasites in the frozen blood group were first detected on day 3 at a low level (0.1%) and rose gradually to 5.0% on day 7.
Conclusions: In conclusion, this finding indicates the fresh blood contains more active and viable parasites, enabling them to rapidly replicate within the mice's bodies.
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DOI: https://doi.org/10.33992/meditory.v13i2.4315
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