PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI EKSTRAK ETANOL DAUN BIDURI SECARA IN VITRO

nyoman mastra

Abstract


ABSTRACT

Background Staphylococcus aureus is a pathogenic bacteria that can cause infection. Biduri leaf has antibacterial compound such as tannin, flavonoid, saponin, and polyphenol.

Objective This study aimed to know the difference of growth inhibition zone of Staphylococcus aureus at the various concentration of ethanol extract of biduri leaf and to determine an effective concentration.

Methods This study was the true experiment with posttest only control design, used Kirby-Bauer disk diffusion method with five concentrations (20%, 40%, 60%, 80%,100%), positive control (chloramphenicol 30 µg) and negative control (ethanol 96%).

Result The result of this study showed the average of inhibition zone diameter of each concentration consecutively 26,2 mm, 28,3 mm, 29,7 mm, 31 mm and 31,5 mm. One Way Anova statistic analysis showed that the value of p is 0.000 so there is the difference of growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of a biduri leaf.

Conclusion The conclusion of this study is there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of biduri leaf and an effective concentration that can inhibit of Staphylococcus aureus is concentration 20% with mean inhibition zone is 26,2 mm.

 

Keywords: ethanol extract of biduri leaf; Staphylococcus aureus; inhibition zone


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References


Jawetz, Melnick and Adelberg. Mikrobiologi Kedokteran. Edisi 25. Alih bahasa: Aryandhito Widhi Nugroho. Jakarta: Buku Kedokteran EGC. 2013

Radji, M. Buku Ajar Mikrobiologi Panduan Mahasiswa Farmasi dan Kedokteran. Jakarta: Penerbit Buku Kedokteran EGC. 2011

Herbie, T. Kitab Tanaman Berkhasiat Obat: 226 Tumbuhan Obat Untuk Penyembuhan Penyakit dan Kebugaran Tubuh. Yogyakarta: Octopus Publishing House. 2015

Kumar, G., L. Karthik and K.V.B. Rao. Antibacterial Activity Aqueous Extract of Calotropis gigantean Leaves-An In Vitro Study. International Journal of Pharmaceutical Sciences Review and Research.4(2): 141-144.http://www.globalresearchonline.net/journalcontents/volume4issue2/Article%20024.pdf.diaksestanggal 26 November 2016. 2010

Katzung, B.G. Farmakologi Dasar & Klinik. Edisi 10. Alih Bahasa: Aryandhito Widhi Nugroho. Jakarta: Buku Kedokteran EGC. 2012

Ramadhan, I. Efek Antiseptik Berbagai Merk Hand Sanitizer Terhadap Bakteri Staphylococcus aureus. Skripsi. Fakultas Kedokteran dan Ilmu Kesehatan, Program Studi Pendidikan Dokter, Universitas Islam Negeri Syarif Hidayatullah Jakarta. 2013

Sujatmiko, A.Y. Aktivitas Antibakteri Ekstrak Kayu Manis (Cinnamomum burmannii B.) Dengan Cara Ekstraksi Yang Berbeda Terhadap Escherichia coli Sensitif Dan Multiresisten Antibiotik. Naskah Publikasi. Fakultas Keguruan Dan Ilmu Pendidikan, Program Studi Biologi, Universitas Muhammadiyah Surakarta. 2014

Kee, J.L and Hayes, E.R. Farmakologi: Pendekatan Proses Keperawatan. Alih bahasa: dr. Peter Anugrah. Jakarta: Buku Kedokteran EGC. 2008

Suryani, D. Efektivitas Daun Sukun (Artocarpusaltilis) Dalam Menghambat Pertumbuhan Bakteri Escherichia coli. Karya Tulis Ilmiah. Fakultas Ilmu Kesehatan, UM Palangkaraya. 2014

Kurniawan, B. dan W.F. Aryana. Binahong (Cassia Alata L) As Inhibitor Of Escherichia coli Growth. J Majority. 4(4): 100-104. 2015




DOI: https://doi.org/10.33992/m.v6i1.227

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