UJI AKTIVITAS ANTIBAKTERI REBUSAN DAUN SIRIH (Piper betle Linn) TERHADAP BAKTERI Streptococcus pyogenes
DOI:
https://doi.org/10.33992/m.v7i1.639Abstract
ABSTRACT
Background: Infection is a disease caused by pathogenic microbes and is very dynamic. ISPA is defined as an acute respiratory disease caused by infectious agents that were transmitted from human to human. One of the microbes that caused ISPA is Streptococcus pyogenes, which is a gram-positive bacteria and belongs to Streptococcus sp. β-hemolytic class A. Nowadays, many people are begin to use natural materials to deal with microbial infections. One of them is the betel leaf (Piper betle Linn.) Which has antibacterial and antibiotic components for the treatment of respiratory problems, and bacterial infectious diseases.
Purpose: This research was conducted with the aim to know the antibacterial activity of betel leaves (Piper betle Linn) decoction against Streptococcus pyogenes bacteria. Methods: This research used true experimental design with Posttest Only Control Design, and data analysis with One Way Anova and Least Significant Deference (LSD). Betel leaves decoction water is made in concentrations of 20%, 40%, 60%, 80%, and 100%, then tested on Streptococcus pyogenes bacteria with two replicates and four repetitions.
Result: From a total of 40 data obtained, the mean diameter of the inhibitory zone at each concentrations is 20% 0 mm, 40% 12.9 mm, 60% 22.7 mm, 80% 25.5 mm, and 100% 30 mm.
Conclution: Betel leaves decoction water is able to inhibit the growth of Streptococcus pyogenes with significant difference of drag zone diameter at each concentration.
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Keywords: Antibacterial, Betel Leaves, Streptococcus pyogenes
References
Asriadi. Uji Sensitivitas Beberapa Antibiotika Terhadap Bakteri Penyebab Infeksi Saluran Pernapasan Atas (Ispa) Di Rsud Syech Yusuf Kab. Gowa. 2012. http://repositori.uin-alauddin.ac.id/3203/1/ASRIADI.pdf.
Nizet V, Arnold JC. Streptococcus Pyogenes (Group A Streptococcus). Fourth Edi. Elsevier Inc.; 2012. doi:10.1016/B978-1-4377-2702-9.00120-3.
Patil RS, Harale PM, Shivangekar KV, Kumbhar PP, Desai RR. Phytochemical potential and in vitro antimicrobial activity of Piper betle Linn. leaf extracts. J Chem Pharm Res. 2015;7(5):1095-1101. http://www.jocpr.com/articles/phytochemical-potential-and-in-vitro-antimicrobial-activity-of-piper-betle-linn-leaf-extracts.pdf.
Akbar R. Aneka Tanaman Apotek Hidup Di Sekitar Kita. 1st ed. (Cahyono F, ed.). Jakarta: One Book; 2015.
Sugianti NKM. Pengaruh Air Rebusan Daun Sirih Dalam Berbagai Konsentrasi Terhadap Daya Hambat Pertumbuhan Bakteri Streptococcus mutans Secara In Vitro. 2015.
CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 27th ed. (Tertel ML, Christopher JP, Martin L, Russell MA, eds.). Wayne: Clinical and Laboratory Standards Institute; 2017.
Yanti YN, Mitika S. Uji Efektivitas Antibakteri Ekstrak Etanol Daun Sambiloto ( Andrographis paniculata Nees ) Terhadap Bakteri Staphylococus aureus. J Ilm Ibnu Sina. 2017;2(1):158-168.
Chakraborty D, Shah B. Antimicrobial , Anti  Oxidative and Anti  Hemolytic Activity of Piper Betel Leaf Extracts. Int J Pharm Pharm Sci. 2011;3(3):192-199.
Rohyani IS, Aryanti E, Suripto. Kandungan fitokimia beberapa jenis tumbuhan lokal yang sering dimanfaatkan sebagai bahan baku obat. 2015;1(April):388-391. doi:10.13057/psnmbi/m010237.
Mariyatin H, Widyowati E, Lestari S. Efektivitas antibakteri ekstrak daun sirih merah ( Piper crocatum ) dan sirih hijau ( Piper Betle L . ) sebagai bahan alternatif irigasi saluran akar. e-Jurnal Pustaka Kesehat. 2014;2(3):556-562.
Nivianti D. Efektivitas Infus Daun Sirih Sebagai Antibakteri Streptococcus mutans Penyebab Karies Gigi. Sainmatika. 2013;10(1):7-10.
Utami DER, Krismayanti L, Yahdi. Pengaruh jenis sirih dan variasi konsentrasi ekstrak terhadap pertumbuhan jamur. 2015;VII.
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