POTENSI EKSTRAK KULIT BUAH DELIMA TERHADAP PERTUMBUHAN Candida albicans SECARA IN VITRO
DOI:
https://doi.org/10.33992/m.v5i2.125Abstract
Abstract
Background: Infection by the Candida albicans has increased globally and reported fungal resistance to synthetic antifungal. It is, therefore, necessary to develop effective antifungal medicines for the treatment of infection by Candida albicans.
This study aims to determine the content of phytochemical and inhibitory power of pomegranate skin extract on Candida albicans growth.
Methode: This research type is the true experiment with post test only control design. The method used for the phytochemical test is qualitative tube method. Pomegranate skin resistivity test on Candida albicans growth was done by disc diffusion method. The pomegranate skin is tested at concentrations of 100%, 75%, 50%, 25% and 10%. Negative control with 96% alcohol and positive control using ketocenazole 2%.
Result: The results of phytochemical tests showed pomegranate skin extract containing saponins, tannins, and flavonoids. Pomegranate skin extracts are not able to inhibit the growth of Candida albicans by disc diffusion method.
Conclusion:The conclusion of this study is pomegranate skin extracts can not inhibit Candida albicans growth, For other researchers which use jatropha curcas twigs, are suggested to do a phytochemical test quantitatively and to test the antifungal potential with liquid DMSO 10%, kloroform and petroleum eter.
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Keywords:Potential antifungal, pomegranate skin extract, Candida albicans
References
DAFTAR PUSTAKA
Jawetz, Melnick dan Adelberg. 2012. Mikrobiologi Kedokteran Edisi 25. Alih Bahasa : Geo F. Brooks; Karen C. Caroll; Janet S. Butel; Stephen A.Morse; Timothy A. Mietzner. Jakarta: EGC.
Rezeki, S., Z. Mubarak dan I. Syuhada. 2013. Gambaran Sensitivitas Isolat Candida Albicans Oral Terhadap Nistatin Dan Flukonazol Pada Pasien Diabetes Melitus Tipe 2 Di Rsudza Banda Aceh. Cakradonya Dental Journal. 5(1): 523-533.
Duryatmo, S. 2010. Herbal Indonesia Berkhasiat Bukti Ilmiah & Cara Racik. Jakarta: PT Trubus Swadaya.
Noor, 2011. Metodologi Penelitian, Jakarta: Kencana Group
Hanafiah, K.A. 2005. Rancangan Percobaan Aplikatif, Jakarta: PT Raja Grafindo Persada
Bhaskara, G.Y. 2012. Uji Daya Antifungi Ekstrak Etanol Daun Salam (Syzygium Polianthum [Wight] Walp.) Terhadap CandidaAlbicans Atcc 10231 Secara In Vitro. Tersedia dalam http://onesearch.id/Record/IOS2728.22008?widget=1&repository_id=2554#details. diakses 6 Juni 2017, 18.23 WITA.
Cushnie, T.P., dan A.J. Lamb. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents. 2005; 26(5): 343-56.
Lim, S.H., I. Darah, and K. Jain. Antimicrobial activities of tannins extracted from Rhizophora apiculata Barks. Journal of Tropical Forest Science. 2006; 18(1): 59-65.
Ansari M.A., A. Anurag, Z.Fatima dan S.Hameed. 2013. Natural Phenolic Compounds: A Potential Antifungal Agent.Microbial pathogens and strategies for combating them: science, technology and education. 1(1): 1189: 1195.
Maharani, S., O. Santoso 2012. Pengaruh Pemberian Larutan Ekstrak Siwak (Salvadora persica) pada berbagai konsentrasi terhadap pertumbuhan Candida albicans. Jurnal PDGI. 61(2): 61-64.
Turkey, M. dan Figen. 2006. Saponins versus plant fungal pathogens. Journal of Cell & Molecular Biology. 5(1): 13-17.
Amelia, S. 2011. Obat Anti Jamur (Fungal), tersedia dalam http://repository.usu.ac.id/bitstream/handle/123456789/30412/Kemoterapi%20Anti%`20Jamur.pdf?sequence=1&isAllowed=ydiakses tanggal 19 Juni 2017
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