Wimbuh Tri Widodo Magister of Forensic Science, Postgraduate School, Airlangga University.
Human Genetics Laboratory, Institute of Tropical Disease, Airlangga. Indonesia
Indah Nuraini Masjkur Human Genetics Laboratory, Institute of Tropical Disease, Airlangga University, Campus C Street, Surabaya
Qurrota A’yunil Huda Human Genetics Laboratory, Institute of Tropical Disease, Airlangga University, Campus C Street, Surabaya
Ahmad Yudianto Forensic Science, Postgraduate School, Airlangga University.
Human Genetics Laboratory, Institute of Tropical Disease, Airlangga University
Sonny Kristianto Forensic Science, Postgraduate School, Airlangga University.
Human Genetics Laboratory, Institute of Tropical Disease, Airlangga University
IDENTIFICATION OF HUMAN HAIR USING DIRECT PCR TARGETING THE CYTOCHROME B GENE
Wimbuh Tri Widodo, Indah Nuraini Masjkur, Qurrota A’yunil Huda, Ahmad Yudianto, Sonny Kristianto, Rury Erina Putri
Abstract
Background:Hair is one of the human parts that is easily scattered and easy to obtain for testing. Direct PCR which is PCR without going through the DNA extraction process has been widely used in various fields. The cytochrome b gene is one of the genes located in mitochondrial DNA. The gene is unique in that it has a small variation within one species organism but has a large variation between species. Aims:c. Methods:Hair was heated at 600C for 10 minutes then the sample was used for the PCR template. PCR was performed using the cytochrome b gene. The results:The analysis showed that human hair could still be detected on day 30 using direct PCR using the cytochrome b gene. Conclusions: This result will simplify and save time in analysis in various fields related to hair shafts.
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