ALTERNATIVE MEDIA FOR BACTERIAL GROWTH STAPHYLOCOCCUS AUREUS: SWEET POTATO INFUSA (IPOMOEA BATATAS), SOYBEANS (GLYCINE MAX (L) MERRILL) AND YEAST EXTRACT

Authors

  • Marta Atik Martsiningsih
  • Fardhiasih Dwi Astuti Public Health Faculty, Ahmad Dahlan University, Yogyakarta, Indonesia
  • Sistiyono Sistiyono Medical Laboratory Technology Department, Health Polytechnic of Ministry of Health, Yogyakarta
  • Suyana Suyana Medical Laboratory Technology Department, Health Polytechnic of Ministry of Health, Yogyakarta
  • Ullya Rahmawati Medical Laboratory Technology Department, Health Polytechnic of Ministry of Health, Yogyakarta, Indonesia
  • Muji Rahayu Medical Laboratory Technology Department, Health Polytechnic of Ministry of Health, Yogyakarta, Indonesia
  • Novita Siti Musyafaah Medical Laboratory Technology Department, Health Polytechnic of Ministry of Health, Yogyakarta, Indonesia

DOI:

https://doi.org/10.33992/meditory.v12i1.3081

Keywords:

Staphylococcus aureus, Media Alternatif, Ubi Jalar, Kacang Kedelai, Ekstrak Ragi

Abstract

Background: Staphylococcus aureus needs nourishment for its growth. The needed nutrients of carbohydrates, proteins, nitrogen, vitamins, and minerals from the media. The media commonly used for bacterial growth is the nutrient medium for high prices. Alternative media from natural local foodstuffs are obtained easily and cheaply instead of instant media. This study aims to identify alternative media mixed with infuse sweet potato (ipomoea batatas), soybeans (glycine Max (l) Merrill), and yeast extract that can be used to grow the staphylococcus aureus bacteria.  Methode: this type of research is pure experimental research, and the design used is the posttest-only control design group. Staphylococcus aureus suspension was grown on alternative media and nutrient agar media as a comparison. measurements of colony diameter and number of colonies were compared between alternative media and nutrient agar. Result The results Research has provided an average number of staphylococcus aureus bacteria that grow on alternative media 78.00 x 104 CFU /ml and nutrient media for 94.19 x 104 CFU /ml. Whereas the colony's diameter of alternative media 2.56 mm, and the media nutrient increased by 2.66 mm. Conclusion: Alternative media from a blend of yam, soy infuse, and yeast extract can be used for the growth of the staphylococcus aureus bacteria


Keywords: Staphylococcus aureus; Alternative Media; Potato Infusa; soy infuse; soy infuse

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Published

2024-06-29

Issue

Vol. 12 No. 1 (2024): Meditory, Volume 12 No. 1 Tahun 2024

Section

Research Articles

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