KADAR GLUKOSA PADA SERUM LIPEMIK DENGAN PENGGUNAAN POLYETHYLENE GLYCOL 6000 8% DAN HIGH SPEED SENTRIFUGASI

Martha Atik Martsiningsih, Sujono Sujono, Bambang Supriyanta, Menik Kasiyati, Catherine Surya, Budi Martono, Budi Setiawan

Abstract


Bacground: Lipemic serum is a problem in many laboratories because it can cause the results of glucose levels in the serum to be falsely high. The examination uses a photometric method, which can cause interference with wave lengths and light scattering. As an alternative to high-speed centrifugation to remove lipemic serum, another method for handling lipemic serum is precipitation, which is carried out by processing polyethylene glycol 6000 8%.

Aims: This research wants to know the differences in the handling of lipemic serum before and after being processed using polyethlyene glycol 6000 8% and high-speed centrifugation.

Method: This type of research is an experiment with Static Group Comparison. Research conducted at the Clinical Pathology Laboratory Installation at the Gadjah Mada University Academic Hospital in November 2022. The sample of this study was lipemic serum with a total of twenty samples obtained from the rest of the examination in two Clinical Laboratory Installations Hospitals in Yogyakarta. Research data were analyzed using the Paired T-Test and Independent T-Test. Result: The statistical analysis (p = 0.000) 0.05 revealed that there is a difference in lipemic serum glucose levels before and after PEG 6000 8% processing and high-speed centrifugation. Before processing with polyethylene glycol 6000 8% and high-speed centrifugation, the average yield of glucose levels in lipemic serum was 116.3 mg/dl. The average glucose level before and after PEG 6000 8% processing was 108.65 mg/dl. Before and after high-speed centrifugation, the average glucose level was 114.1 mg/dl.

Conclusion: Overall, both the PEG 6000 8% processing and the high-speed centrifugation resulted in a significant decrease in lipemic serum glucose levels. These methods appear to be effective in reducing the interference caused by lipemia and improving the accuracy of glucose measurements in the serum.


Keywords


Lipemic serum; Polyethylene Glycol 6000 8%; High Speed Centrifugation

Full Text:

PDF

References


Siregar, Maria Tuntun; dkk, 2018, Bahan Ajar Teknologi Laboratorium Medik (TLM) Kendali Mutu, Kemenkes RI, 529 halaman.

Nugraha, Gilang; Badrawi, Immafuddin, 2018. Pedoman Teknik Pemeriksaan Laboratorium Klinik, Jakarta : Penerbit Trans Info Media.

Lieseke, C. L., & A. Zeibig, E. (2014). Buku Ajar Laboratorium Klinis . Jakarta: EGC.

WHO, 2002. Use of Anticoagulants In Diagnostic Laboratory Investigation.

Izzati, A., & Riyani, A. 2018. Variasi Konsentrasi Alfa Siklodekstrin dan Waktu Sentrifugasi Dalam Preparasi Serum Lipemik Pada Pemeriksaan Glukosa Metode GOD-PAP. Jurnal Teknologi Laboratorium. ISSN 2580-0191 (Online). Edisi Maret 2018. Vol. 7, No. 1: 31 – 37. Dalam: https://www.teknolabjournal.com. Diakses pada tanggal 20 Agustus 2022.

Contoins, J.H dan Nguyen, R.A, 2013. Assay Interference: A Need for Increased Understanding and Testing. Sun Diagnostic.

Cynthia M, Roberts, & Steven, W. C. (2013). Cyclodextrin Removal of Lipemic Interference: An Attractive Alternative to Ultracentrifugation for Satellite Laboratories. Archives of Pathology & Laboratory Medicine, 137(8), 1027–1028.

Smith, M. B., Yung W., C., Dolci, A., Kellogg, M. D., McCudden, C. R., McLean, M., Miller, J. J., & Zakowski, J. (2012). Hemolysis, icterus, and lipemia/ turbidity indices as indicators of interference in clinical laboratory analysis; Approved Guideline, CLSI document C56-A, Wayne, PA : Clinical and Laboratory Standards Institute. September, 42.

Soleimani, N., Mohammadzadeh, S., & Asadian, F. (2020). Lipemia Interferences in Biochemical Tests, Investigating the Efficacy of Different Removal Methods in comparison with Ultracentrifugation as the Gold Standard. Journal of Analytical Methods in Chemistry, 2020. https://doi.org/10.1155/2020/9857636.

Direktorat Laboratorium Kesehatan Departemen Kesehatn RI, 2004 Pedoman praktek Laboratorium yang Benar (Good Laboratory Practice), Cetakan ke-3, Jakarta.

Sari, Wheny Mufita. Hardisari,Ratih Ni. Sujono. 2017.Perbedaan kadar Kreatinin pada Y Serum Lipemik yang diolah dengan Polyethylene Glicol 6000 8% dan High Speed Sentrifugasi.Jurnal Kesehatan Volume 13 No 1

Calmarza P. dan Cordero J. 2011. Lipemia Interferences in Routine Clinical Biochemiae Test, Biochemia Medica 2011; 21 (2): 160-6.

Beta lutfi Ayu nastiti 2019 .Perbedaan kadar Protein Total pada serum lipemik yang dipreparasi menggunkan Poliethylen Glikol 6000.8% dengan inkubasi pada suhu 4oC, Suhu ruang dan tanpa inkubasi..Poltekkes Tanjungkarang 2019.

Sari, Wheny Mufita Hardisari, Ni Ratih, Sujono. 2017. Perbedaan Kadar Kreatinin Pada Serum Lipemik Yang Diolah Dengan Polyethylene Glycol High Speed 6000 8% Dan Sentrifugasi. Jurnal Teknologi Kesehatan.

Sujono; Maulida, Yumna; Sari, Melianda, Puspita. 2016. Kadar Protein Total dan Ureum Dengan dan Tanpa Penambahan ℽ-cyclodextrin Pada Serum Lipemik. Jurnal Analis Kesehatan Poltekkes Kemenkes Yogyakarta.

Piyophirapong, S.,W. Wongtiraporn dan K. Sribhen. 2010. Factitious Result in Clinical Chemistry Test Caused by Common Endogenous Interferents.

Niranata, Sistiyono, Setiawan. 2017. Serum Lipemik Dengan Flokulan Gamma-Siklodekstrin Pada Pemeriksaan Glukosa. Jurnal Kesehatan Vol. 10 No. 2.

Neldawati, Ratnawulan, & Gusnedi. (2013). Nilai Absorbansi dalam Penentuan Kadar Flavonoid untuk Berbagai Jenis Daun Tanaman Obat. Pillar Of Physics , 2, 76.

Aryani, T. 2021. Evaluasi Pengolahan Serum Lipemik terhadap Pemeriksaan Kadar Kolesterol Total dan Trigliserida. Journal Ilmiah Analis Kesehatan Vol 7, Issue 2.

Boisrame, J. - Helms, F. Toti, M. Hasselmann, F. Meziani.2015. Lipid Emulsions for Parenteral Nutrition in Critical Illness, Prog Lipid Res. Journa of Biochemia Medical. Vol. 60: No. 1-6. http://dx.doi.org/10.1016/j.plipres.2015.08.002.

Castro, MJ, et al. 2018. Removing Lipemia in Serum/ Plasma Samples, Ann Lab Med J.

Fell, G.L., P. Nandivada, K.M. Gura, M. Puder. 2015. Intravenous Lipid Emulsions in Parenteral Nutrition, Adv Nutr 6(5). http://dx.doi.org/10.3945/an.115.009084. Diakses pada : 15 Agustus 2022.

Ji JZ, Meng QH. Evaluation of the interference of hemoglobin, bilirubin, and lipids on Roche Cobas 6000 assays. Clin Chim Acta. 201; 412(17-18): 1550–3.

Lestari,Estu. Chairlan.2011. Pedoman Teknik Dasar Untuk Laboratorium Kesehatan. EGC,Jakarta.

Nicolac Nora, 2013. Lipemik: Causes, Interference Mechanism Detechon and Mannagement. Biochem Medika. Krosia: University Departement of Chemistry.

Rimadianti, D.M.A. 2008. The Cholesterol Myth. Washington, D.C: New Trend Publishing.

Susiwati S. Perbedaan kadar glukosa darah puasa pasien diabetes melitus tipe 2 pada plasma naf berdasarkan waktu pemeriksaan di rsud dr. M. Yunus provinsi bengkulu tahun 2017. J Nurs Public Heal. 2018;6(1):82-7.

Sukorini, Usi, Nugroho, D.K., Rizki, M., Hendriawan P.J., B. 2010. Pemantapan Mutu Internal Laboratorium Klinik. Yogyakarta: Kanalmedika dan alfamedia Citra.




DOI: https://doi.org/10.33992/meditory.v11i1.2425

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Meditory

POLTEKKES KEMENKES DENPASAR
Address: Jl. Sanitasi No.1, Sidakarya, Denpasar Selatan, Kota Denpasar, Bali 80224 (0361) 710447