Staphylococcus Aureus Bacteria Identification And Sensitivity Test To Amoxicillin Antibiotics On Nurses' Hands In Mangusada Hospital

Made Witari Nugraha Putri, I Gusti Ayu Sri Dhyanaputri, Ni Kadek Wid Cahya Paramita

Abstract


Background: HAIs (Healthcare-Associated Infections), also called nosocomial infections, occur in connection with health services in hospitals. Nosocomial infections can be transmitted or acquired through health workers, sick people, visitors with career status, or because of hospital conditions. One of the treatments for nosocomial infections is the administration of antibiotics. Treatment for uncomplicated nosocomial infections is penicillin; antibiotics belonging to the penicillin group are Amoxicillin. This research aims to identify and determine the sensitivity of Staphylococcus aureus bacteria to amoxicillin antibiotics isolated from a nurse's hand swab sample in the janger room of Mangusada Badung Hospital. This study used a descriptive method and used 20 samples with a purposive sampling technique. Hand swabs were isolated on MSA media and then identified by gram staining, catalase, and coagulase tests. The identification results showed that 10 (55.56%) of 20 samples were Staphylococcus aureus bacteria with the characteristics of coccus, gram-positive, catalase test, and coagulation test positive. This study concluded that the sensitivity test showed that all isolates of Staphylococcus aureus isolates were resistant to Amoxicillin. Results based on this study show that Amoxicillin is not practical in inhibiting the growth of Staphylococcus aureus and is not an appropriate choice for treating nosocomial infections.


Keywords


Staphylococcus aureus, Sensitivity, Amoxicillin

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References


Adiputra, M. S., Ni, W. T., & Ni, P. W. O. (2021). Metodologi Penelitian Kesehatan. Angewandte Chemie International Edition, 6(11), 951–952.

Fajardo, M., Hidalgo, R., Gaitán, J., Sánchez-Silos, R., & Martín-Cordero, P. (2016). About microbiological methods for detection of oxacillin resistance in coagulase-negative staphylococci. Revista Espanola de Quimioterapia : Publicacion Oficial de La Sociedad Espanola de Quimioterapia, 29(5).

Fridalni, N., & Rahmayanti, R. (2020). Faktor-Faktor Yang Berhubungan Dengan Perilaku Perawat Dalam Penggunaan Alat Pelindung Diri. Jurnal Kesehatan Medika Saintika, 11(1). https://doi.org/10.30633/jkms.v11i1.503

Gifari, Z. Al, Anwar, K., Rosyidi, A., Ali, M., & Amin, M. (2022). Isolasi dan Identifikasi Bakteri Asam Laktat Penghasil Enzim Fitase Sebagai Kandidat Probiotik Untuk Ternak Unggas. Jurnal Ilmiah Indonesia, 7(8.5.2017).

Hayati, Z., Azwar, & Puspita, I. (2012). Pattern and Antibiotics’ Sensitivity of Bacteria Potentially Causing Nosocomial Infection at Surgical Wards, RSUDZA, Banda Aceh. Jurnal Kedokteran Yarsi, 20(3).

Juliana, M., M.G Sianipar, I., & Aditya Willyanto, N. (2023). Analysis Of Hand Hygiene Practices Among Health Care Workers For Prevention Of Nosocomial Infection: A Systematic Review. Journal of Advance Research in Medical & Health Science (ISSN: 2208-2425), 9(6). https://doi.org/10.53555/nnmhs.v9i6.1739

Kemenkes RI. (2017). Peraturan Menteri Kesehatan Republik Indonesia Nomor 27 Tahun 2017 Tentang Pedoman Pencegahan Dan Pengendalian Infeksi Di Fasilitas Pelayanan Kesehatan. Kementrian Kesehatan RI.

Leboffe, M. J., & Pierce, B. E. (2013). A Photographic Atlas For The Microbiology for the Laboratory 4th EDITION. In Morton Publishing Company (Vol. 8, Issue 6).

Malau, S. E., & Simanjuntak, S. M. (2019). The Hand Hygiene And Bacterial Colonies Count Of ICCU’ Visitors Hands: As An Indicator For Nosocomial Infection Prevention. Klabat Journal of Nursing, 1(1). https://doi.org/10.37771/kjn.v1i1.381

Murdiana, H. E. (2021). The identification of drug related problems (DRPs) using profilaxis antibiotics in orthopedic surgical patients at a Government Hospital in Yogyakarta. Jurnal Ilmiah Farmasi, 17(2). https://doi.org/10.20885/jif.vol17.iss2.art10

Nursalam. (2011). Manajemen Keperawatan Aplikasi dalam Praktik Keperawatan Profesional Edisi 3. In Salemba Medika.

Organisation, W. H. (2017). WHO Guidelines on Hand Hygiene in Health Care. World Health Organization, 30(1).

Rambiko, Fatmawali, B. (2016). Uji Sensitivitas Bakteri Penyebab Infeksi Nosokomial Saluran Kemih Akibat Penggunaan Kateter Terhadap Antibiotik Ampicillin, Amoxicillin Dan Ciprofloxacin. Pharmacon, 5(1).

Rista, U. N., Scipio, M. V., & Ariani, D. (2022). Kenali Penggunaan Antibiotik Secara Tepat. Kami Mengabdi, 1(2). https://doi.org/10.52447/km.v1i2.6037

Rsud, M., Soewondo, D. H., Windyastuti, K., Kadek, N., Widyastuti, A., Kustriyani, M., Program, D., Ners, S., Semarang, W. H., Program, M., Widya, S., & Semarang, H. (2020). Hubungan Kepatuhan Cuci Tangan Enam Langkah Lima Momen Dengan Kejadian Infeksi Nosokomial Di Ruang Mawar Rsud Dr.H.Soewondo Kendal. Proceeding Widya Husada Nursing Conference, 1(1).

Soleha, T. U. (2015). Uji Kepekaan terhadap Antibiotik Susceptibility Test of Antimicroba. Juke Unila, 5(9).




DOI: https://doi.org/10.33992/icmahs.v1i1.3059

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