IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS WITH R-PCR (REAL TIME POLYMERASE CHAIN REACTION) TECHNIQUE FOR DIAGNOSIS OF PULMONARY TB PATIENTS BALKESMAS SEMARANG

Abstract

Tuberculosis is one of the causes of death due to infection and is transmitted globally by more than 10 million people. Pulmonary tuberculosis (TB) is an infectious disease which is a priority health problem in developing countries, including Indonesia. 16 countries accounted for 93% of tuberculosis cases with 3 countries contributing the most tuberculosis cases namely India, Indonesia and the Philippines. It is very important to develop methods to increase the sensitivity and specificity of TB examination results using the r-PCR (real time polymerase chain reaction) technique. This process requires a double-stranded DNA template containing target DNA, DNA polymerase enzyme, triphosphate nucleotides, and a pair primer.which has better accuracy and precision by using IS6 and IS7 primer. To determine the sensitivity and specificity of the R-PCR (REAL TIME POLYMERASE CHAIN REACTION) TECHNIQUE method. This research is non-experimental analytic in nature. The sample used in this study was a pure culture of Mycobacterium tuberculosis growth in the National Health Service Office of Central Java Province with microscopic testing AFB staining, R-PCR ( REAL TIME POLYMERASE CHAIN REACTION). The results showed that there was no difference between the conventional culture identification method as the standard for identification of Mycobacterium tuberculosis and the results of RT PCR examination with IS6110 primer having 100% sensitivity and specificity.

Kata Kunci : Mycobacterium tuberculosis, kultur , RT – PCR