Antimicrobial Resistance Test of Elephant Ginger (Zingiber officinale var. Officinarum) Ethanol Extract Against Streptococcus pyogenes Bacteria Using the Disc Diffusion Method
Abstract
Background of this study is acute respiratory tract infection (ARI) caused by the bacterium
Streptococcus pyogenes. The use of antibiotics is not always effective due to the emergence of
resistance. Therefore, natural treatment alternatives such as elephant ginger, which contains active
compounds like tannins, saponins, phenols, alkaloids, and flavonoids, are needed. Purpose of this study
to measure the diameter of the inhibition zone, analyze, and categorize the effect of ethanol extract of
elephant ginger on the growth of Streptococcus pyogenes. Research method is a true experimental
design with Posttest Only Control Design, and data analysis with One Way ANOVA and Least Significant
Deference (LSD). The ethanol extract of elephant ginger was prepared in concentrations of 15%, 17.5%,
20%, and 22.5%, and tested against Streptococcus pyogenes bacteria with three repetitions. Results
showed that the inhibition zone increased with the volume of extract used. The 15% concentration
produced 8.83 mm (moderate), 17.5% resulted in 9.08 mm (moderate), 20% in 10.52 mm (strong), and
22.5% in 11.06 mm (strong). The conclusion of this study is that there is a significant difference in the
inhibition zone diameters of Streptococcus pyogenes growth at each concentration of 15%, 17.5%, 20%,
and 22.5%.
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PDFReferences
Badan Penelitian dan Pengembangan Kesehatan. (2018). Laporan nasional Riskesdas 2018.
Kementerian Kesehatan Republik Indonesia.
Sari, D., & Nasuha, A. (2021). Kandungan zat gizi, fitokimia, dan aktivitas farmakologis pada jahe
(Zingiber officinale Rosc.): Review. Tropical Bioscience: Journal of Biological Science, 1(2), 11–
Tobat, S. R., Mukhtar, M. H., & Pakpahan, I. H. D. (2015). Rasionalitas penggunaan antibiotika pada
penyakit ISPA di Puskesmas Kuamang Kuning I Kabupaten Bungo. Scientia: Jurnal Farmasi dan
Kesehatan, 5(2), 79.
Rostinawati, T. R., Rikadianan, R., & Wicaksono, I. A. (2022). Sensitivity assay of resistant Escherichia
coli to fosfomycin, kanamycin, rifampicin, ceftriaxone, imipenem, and vancomycin. Indonesian
Journal of Pharmaceutical Science and Technology, 1(1), 1.
Newman, D. J., & Cragg, G. M. (2020). Natural products as sources of new drugs over the nearly four
decades from 01/1981 to 09/2019. Journal of Natural Products, 83(3), 770–803.
https://doi.org/10.1021/acs.jnatprod.9b01285
Dianawati, N., & Manisha, R. (2023). Perbedaan antara ekstrak etanol jahe emprit (Zingiber officinale
var. amarum), jahe gajah (Zingiber officinale var. officinarum), dan jahe merah (Zingiber officinale
var. rubrum) dalam menghambat bakteri Streptococcus mutans. Bhakta Dental Journal, 1(1), 13–
Fibryanto, E., Stefani, R., & Winaldy, B. (2022). Pengaruh ekstrak jahe gajah (Zingiber officinale var.
officinarum) terhadap jumlah koloni Streptococcus mutans (in vitro). Jurnal Kedokteran Gigi
Universitas Padjadjaran, 34(2), 136–142.
Rostinawati, T. R., Rikadianan, R., & Wicaksono, I. A. (2022). Sensitivity assay of resistant Escherichia
coli to fosfomycin, kanamycin, rifampicin, ceftriaxone, imipenem, and vancomycin. Indonesian
Journal of Pharmaceutical Science and Technology, 1(1), 1.
Dwijastuti, N. M. S., & Dewi, G. A. A. S. (2023). Perbedaan hasil kultur anaerob dengan candle jar dan
gas generating sachet untuk bakteri asam laktat Lactobacillus plantarum E12.1. Jurnal Biologi
Makassar, 8(1), 92–101.
Sumilat, D. A. (2019). Antibacterial screening activity of several sponges against Staphylococcus aureus,
Escherichia coli, Staphylococcus saprophyticus, dan Pseudomonas aeruginosa. Jurnal Ilmiah
Platax, 7(2), 455.
Herawati, I. E., & Saptarini, N. M. (2020). Studi fitokimia pada jahe merah (Zingiber officinale Roscoe var.
sunti Val). Majalah Farmasetika, 4(Suppl. 1), 22–27.
Alouw, G., Fatimawali, F., & Lebang, J. S. (2022). Uji aktivitas antibakteri ekstrak etanol daun kersen
(Muntingia calabura L.) terhadap bakteri Staphylococcus aureus dan Pseudomonas
aeruginosa dengan metode difusi sumuran. Jurnal Farmasi Medica/Pharmacy Medical Journal
(PMJ), 5(1), 36.
Utami, D. E. R., Krismayanti, L., & Yahdi, Y. (2018). Pengaruh jenis sirih dan variasi konsentrasi ekstrak
terhadap pertumbuhan jamur Candida albicans. Biota, 8(2), 142–156.
Magvirah, T., Marwati, M., & Ardhani, F. (2020). Uji daya hambat bakteri Staphylococcus aureus
menggunakan ekstrak daun tahongai (Kleinhovia hospita L.). Jurnal Peternakan Lingkungan
Tropis, 2(2), 41.
Sitepu, J. N. (2023). Uji aktivitas antibakteri ekstrak jahe merah (Zingiber officinale var. rubrum) dan
ekstrak bawang putih (Allium sativum) terhadap Streptococcus pyogenes dengan metode difusi
cakram. Nommensen Journal of Medicine, 8(2), 77–81.
Wilapangga, A., & Syaputra, S. (2018). Analisis antibakteri metode agar cakram dan uji toksisitas
menggunakan BSLT (brine shrimp lethality test) dari ekstrak metanol daun salam (Eugenia
polyantha). Indonesian Journal of Biotechnology and Biodiversity, 2(2), 50–56.
DOI: https://doi.org/10.33992/icmahs.v3i1.4575
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