HASIL PEMERIKSAAN IMUNOKROMATOGRAFI COACTAIL ANTIGEN M.TUBERCULOSIS DAN METODE MOLEKULER PADA SUSPEK HIV

Rizana Fajrunni'mah

Abstract


Background Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) infection. Tuberculosis is one of the most common infections in people with HIV/ AIDS. Deaths due to TB in HIV patients occur due to delayed diagnosis. GeneXpert is a breakthrough discovery for TB diagnosis based on molecular examination using the semi quantitative Real Time Polymerase Chain Reaction Assay (RT-PCR) method. An examination has now been developed to detect MTB antigens using the method of rapid immunochromatography in the hope that it can be used as a means of supporting TB diagnosis better than detection of antibodies to MTB.

Aim This study aims to compare the results of the TB GeneXpert RTPCR method with the rapid immunochromatography method in suspect HIV.

Method This study uses a quantitative design with observational analytic design (cross sectional) with primary and secondary data collection.

Result The results of this study found the value of sensitivity, specificity, accuracy, positive predictive value, negative predictive value of the ICT test in a row of 21%, 76%, 40%, 55.8%.

Conclusion ICT TB has low sensitivity and sufficient specificity so that it is still not good if used for initial screening to detect pulmonary TB in suspect HIV.

 


Keywords


Tuberculosis, HIV, moleculer method, rapid immunochromatography method

Full Text:

PDF

References


World Health Organisation. Global tuberculosis report. Geneva: World Health Organisation. 2013.

World Health Organisation. Global tuberculosis report. Geneva: World Health Organisation. 2014.

Badan Penelitian dan Pengembangan Kesehatan. Riset kesehatan dasar. Jakarta: Kementerian Kesehatan. 2013.

Mulyadi, Fitrika Y. “Hubungan Tuberkulosis Dengan HIV/AIDS”. Idea Noursing Journal Vol.2, No.2, p:162-166, 2011.

Salwani D. Kemampuan gabungan gejala klinis dan pemeriksaan laboratorium serta BACTEX MGIT 960 dalam diagnosis tuberkulosis pada suspek HIV. [tesis]. Jakarta: Fakultas Kedokteran Universitas Indonesia, 2012.

Schwander, Ellner JJ. The Human Host: Immunology and Susceptibility. In: Mario R, editor. Tuberculosis: A Comprehensive International Approach. London: Informa Health Care. p. 117-54, 2006.

Domingo JP. Tuberculosis and HIV/AIDS. In: Palomino, editor. Tuberculosis 2007 from Basic Science to Patient Care. Brazilia: Bernd Sebastian Kamps and Patricia Bourcillier, p. 559- 85, 2007.

Sinaga, H. Isolasi dan Identifikasi Mycobacterium tuberculosis untuk Petugas Laboratorium. Palembang: Multi Sarana, 2011.

Parsons LM, et al. “Laboratory Diagnosis of Tuberculosis in Resource-Poor Countries: Challenges and Opportunities”. Clinical Microbiology Reviews, Vol. 24, No. 2, pp.314-350, 2011.

Lyanda A. “Rapid TB test”. Jurnal Tuberkulosis Indonesia. Vol.8, pp:12-17, 2012.

Wulandari Y, Wiqoyah N, Mertaniasih NM. “Nucleicacid amplification of the RPOB region ofMycobacterium tuberculosis in pulmonarytuberculosis diagnosis”. Folia Medica Indonesiana Vol 47(4), pp 224-229, 2011.

Afrilyantina NI, dkk. “Kemampuan Diagnostik Pemeriksaan Xpert MTB/RIF® dengan Acuan Kultur Media Cair pada Suspek HIV”. Ina J CHEST Crit and Emerg Med. Vol. 2, No.3, pp 118-122, 2015.

Weyer K, Mirzayev F, Migliori, Gemert, et al. “Rapid molecular TB diagnosis: evidence, policy making and global implementation of Xpert MTB/RIF”. European Respiratory Journal, Vol 42, pp 252-271, 2013

Mathur ML, LoBue PA, & CatanzaroA. 1999. Evaluation of a serologic test for the diagnosis of tuberculosis. Int J Tuberc Lung Disc, Vol 3(8), pp 732-5, 1999.

Amin Z, Uyainah A, Yunihastuti E, Djoerban Z. “Profil Pasien TbHiv Dan Non Tb-Hiv Di RSCM”. Bul Penelit Kesehat. Vol 41(4), pp 195–9, 2013

Soraya D, Artika DM. “Profil Pasien Koinfeksi TB-HIV Di Rumah Sakit Umum Pusat Sanglah Bali Tahun 2013”. EJURNAL Med, Vol 5(20), pp 66– 71, 2016.

Widiyanti M, Fitriana E, Iriani E. “Karakteristik Pasien Koinfeksi TB-HIV Di Rumah Sakit Mitra Masyarakat Mimika Papua”. SEL, Vol. 3 No. 2, pp 49-55, 2016.

Pusat Data dan Informasi Kementerian Kesehatan RI. Situasi Umum HIV/AIDS dan Tes HIV. 2018. http://www.pusdatin.kemkes.go.id/article/view/17020100001/situasi-penyakit-hiv-aids-di-indonesia.html

Permitasari. Faktor risiko terjadinya koinfeksi tuberkulosis pada pasien hiv/aids di rsup dr. Kariadi semarang. KTI. Pendidikan Kedokteran Universitas Diponegoro. 2012.

Jayakody W, Harries A.D, Malhotra S, Alwis S, Samaraweera S, Pallewatta N. “Characteristics And Outcomes Of Tuberculosis Pattients Who Fail To Smear Convert At Two Months In Sri Lanka”. PHA, Vol 3(1), pp 26-30, 2013.




DOI: https://doi.org/10.33992/m.v8i1.1093

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Meditory : The Journal of Medical Laboratory

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Meditory